Detection and processing of peripheral myelin protein PMP22 in cultured Schwann cells
نویسندگان
چکیده
منابع مشابه
Cultured Schwann Cells Constitutively Express the Myelin Protein P0
It is widely thought that mammalian Schwann cells do not express Po, the major glycoprotein in peripheral myelin, unless they are induced to do so by axonal signals that can be mimicked by agents that trigger cAMP signaling pathways. In contrast, we find that cultured Schwann cells make large amounts of Po without the addition of any axonal-like signal, provided they have not been exposed to se...
متن کاملDevelopmental changes in myelin-induced proliferation of cultured Schwann cells
Schwann cell proliferation induced by a myelin-enriched fraction was examined in vitro. Although nearly all the Schwann cells contained material that was recognized by antisera to myelin basic protein after 24 h, only 1% of the cells were synthesizing DNA. 72 h after the addition of the mitogen a maximum of 10% of the cells incorporated [3H]thymidine. If the cultures were treated with the myeli...
متن کاملUncoupling of myelin assembly and schwann cell differentiation by transgenic overexpression of peripheral myelin protein 22.
We have generated previously transgenic rats that overexpress peripheral myelin protein 22 (PMP22) in Schwann cells. In the nerves of these animals, Schwann cells have segregated with axons to the normal 1:1 ratio but remain arrested at the promyelinating stage, apparently unable to elaborate myelin sheaths. We have examined gene expression of these dysmyelinating Schwann cells using semiquanti...
متن کاملNeurons promote the translocation of peripheral myelin protein 22 into myelin.
Schwann cells express low levels of myelin proteins in the absence of neurons. When Schwann cells and neurons are cultured together the production of myelin proteins is elevated, and myelin is formed. For peripheral myelin protein 22 (PMP22), the exact amount of protein produced is critical, because peripheral neuropathies result from its underexpression or overexpression. In this study we exam...
متن کاملPeripheral myelin protein-22 (PMP22) modulates alpha 6 integrin expression in the human endometrium
BACKGROUND PMP22, a member of the GAS3 family of tetraspan proteins, is associated with a variety of neurological diseases. Previous studies have shown that PMP22 is expressed in proliferative endometrium, but its function within this tissue is poorly understood. In this study, we first characterized the expression of PMP22 in the human menstrual cycle and began to characterize its function in ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1993
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)82211-6